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Cell Line Services

To provide maximum antibody yield during production, SDI examines the viability of your cell line and the clonality of your cell line. Clonality is the determination of the fraction of cells secreting antibody in a culture. To achieve maximum antibody yield during production resulting in a lower price per milligram of antibody, each cell line is evaluated in three steps:

Recovery: We assess the viability of the hybridoma cells in the cryovial you supply to us. If the culture viability is low, we attempt to recover viability cells and establish a healthy culture. If the culture viability is acceptable at thaw, recovery work is not required and we move directly to Clonality testing. Once a healthy culture is established, the clonality of the cell line is determined.

Clonality: The fraction of cells secreting monoclonal antibody (the clonality) in a healthy culture is determined. Monoclonal antibody yield is improved when the clonality of the cell line is high. Failing to determine the clonality of the cell line prior to production may lead to unexpectedly low yields of monoclonal antibody.

Subcloning: If the clonality of the culture is acceptable, the original culture is used for production work or cell banking work. If the clonality of the culture is unacceptable, SDI subclones the original culture by selecting the best antibody-secreting clone. This subclone is used for production or cell banking.

Cell Banking:

A cell bank provides a stable, uniform source of hybridoma cells for monoclonal antibody production. Your cell line is used for generation of a Master Cell Bank (MCB) and a Manufacturer's Working Cell Bank (MWCB). Before freezing the cell bank, the clonality of your cell line is determined and isotype and isoelectric point of the monoclonal antibody is determined. After the cell bank is frozen, cryovials are thawed and the same tested are performed on the cells in the post-freeze culture and the monoclonal antibody. When post-freeze testing is completed, the cell bank undergoes mycoplasma and sterility testing.

Cell Line Service Pricing

Complete and fax the Custom Monoclonal Project Submission form, then send us the signed original with your antigen.

Full ordering instructions here.